Internal epitope tagging informed by relative lack of sequence conservation.

ABSTRACT: Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.

SUBMITTER: Burg L 

PROVIDER: S-EPMC5125009 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature