Impaired function of trophoblast cells derived from translocated hESCs may explain pregnancy loss in women with balanced translocation (11;22).

ABSTRACT:

Purpose

The aim of the study was to study whether the trophoblasts carrying unbalanced translocation 11,22 [t(11;12)] display abnormal expression of trophoblastic genes and impaired functional properties that may explain implantation failure.

Methods

t(11;22) hESCs and control hESCs were differentiated in vitro into trophoblast cells in the presence of BMP4, and trophoblast vesicles (TBVs) were created in suspension. The expression pattern of extravillous trophoblast (EVT) genes was compared between translocated and control TBVs. The functional properties of the TBVs were evaluated by their attachment to endometrium cells (ECC1) and invasion through trans-well inserts.

Results

TBVs derived from control hESCs expressed EVT genes from functioning trophoblast cells. In c

SUBMITTER: Shpiz A 

PROVIDER: S-EPMC5125145 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature