Genistein Induces Apoptosis and Inhibits Proliferation of HT29 Colon Cancer Cells.

ABSTRACT: Soybean isoflavone genistein has multiple anticancer properties and its pro-apoptotic and anti-proliferative effects have been studied in different cancer cells. However, the mechanisms of action of genistein and its molecular targets on human colon cells have not been fully elucidated. Therefore, caspase-3 and p38 mitogen-activated protein kinase (p38 MAPK) as the main therapeutic targets were investigated in this study at both gene expression and protein levels in HT29 colon cancer cells. The caspase-3 and p38 MAPK gene expression levels were examined by real time PCR whereas flow cytometry technique was performed to determine their intracellular protein levels. The caspase-3 enzyme activity was obtained by colorimetric method while the gelatinase activity of matrix metalloprotein

SUBMITTER: Shafiee G 

PROVIDER: S-EPMC5125370 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature