Integration of growth factor gene delivery with collagen-triggered wound repair cascades using collagen-mimetic peptides.

ABSTRACT: Growth factors (GFs) play vital roles in wound repair. Many GF therapies have reached clinical trials, but success has been hindered by safety concerns and a lack of efficacy. Previously, we presented an approach to produce protein factors in wound beds through localized gene delivery mediated by biomimetic peptides. Modification of polyethylenimine (PEI) DNA polyplexes with collagen-mimetic peptides (CMPs) enabled tailoring of polyplex release/retention and improved gene transfer activity in a cell-responsive manner. In this work, CMP-mediated delivery from collagen was shown to improve expression of platelet-derived growth factor-BB (PDGF-BB) and promote a diverse range of cellular processes associated with wound healing, including proliferation, extracellular matrix production, and chem

SUBMITTER: Urello MA 

PROVIDER: S-EPMC5125401 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature