Controlling the surface-mediated release of DNA using 'mixed multilayers'.

ABSTRACT: We report the design of erodible 'mixed multilayer' coatings fabricated using plasmid DNA and combinations of both hydrolytically degradable and charge-shifting cationic polymer building blocks. Films fabricated layer-by-layer using combinations of a model poly(β-amino ester) (polymer 1) and a model charge-shifting polymer (polymer 2) exhibited DNA release profiles that were substantially different than those assembled using DNA and either polymer 1 or polymer 2 alone. In addition, the order in which layers of these two cationic polymers were deposited during assembly had a profound impact on DNA release profiles when these materials were incubated in physiological buffer. Mixed multilayers ∼225 nm thick fabricated by depositing layers of polymer 1/DNA on

SUBMITTER: Appadoo V 

PROVIDER: S-EPMC5125402 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature