A unified approach towards Trypanosoma brucei functional genomics using Gibson assembly.

ABSTRACT: Trypanosoma brucei is the causative agent of human African trypanosomiasis and nagana in cattle. Recent advances in high throughput phenotypic and interaction screens have identified a wealth of novel candidate proteins for diverse functions such as drug resistance, life cycle progression, and cytoskeletal biogenesis. Characterization of these proteins will allow a more mechanistic understanding of the biology of this important pathogen and could identify novel drug targets. However, methods for rapidly validating and prioritizing these potential targets are still being developed. While gene tagging via homologous recombination and RNA interference are available in T. brucei, a general strategy for creating the most effective constructs for these approaches is lacking. Here, we adapt Gibso

SUBMITTER: McAllaster MR 

PROVIDER: S-EPMC5125862 | biostudies-literature | 2016 Nov - Dec

REPOSITORIES: biostudies-literature