Primer Sets Developed for Functional Genes Reveal Shifts in Functionality of Fungal Community in Soils.

ABSTRACT: Phylogenetic diversity of soil microbes is a hot topic at the moment. However, the molecular tools for the assessment of functional diversity in the fungal community are less developed than tools based on genes encoding the ribosomal operon. Here 20 sets of primers targeting genes involved mainly in carbon cycling were designed and/or validated and the functioning of soil fungal communities along a chronosequence of land abandonment from agriculture was evaluated using them. We hypothesized that changes in fungal community structure during secondary succession would lead to difference in the types of genes present in soils and that these changes would be directional. We expected an increase in genes involved in degradation of recalcitrant organic matter in time since agriculture. Out of th

SUBMITTER: Hannula SE 

PROVIDER: S-EPMC5126076 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature