The degradation of nucleotide triphosphates extracted under boiling ethanol conditions is prevented by the yeast cellular matrix.

ABSTRACT:

Introduction

Boiling ethanol extraction is a frequently used method for metabolomics studies of biological samples. However, the stability of several central carbon metabolites, including nucleotide triphosphates, and the influence of the cellular matrix on their degradation have not been addressed.

Objectives

To study how a complex cellular matrix extracted from yeast (Saccharomyces cerevisiae) may affect the degradation profiles of nucleotide triphosphates extracted under boiling ethanol conditions.

Methods

We present a double-labelling LC-MS approach with a ¹³C-labeled yeast cellular extract as complex surrogate matrix, and ¹³C¹⁵N-labeled nucleotides as internal standards, to study the effect of the yeast matrix on the degrad

SUBMITTER: Gil A 

PROVIDER: S-EPMC5126204 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature