AGE/RAGE promotes thecalcification of human aortic smooth muscle cells via the Wnt/β-catenin axis.

ABSTRACT:

Objective

The present study aims to investigate whether RAGE promotes the calcification of human arterial smooth muscle cells (HASMCs) and determine the relationshipbetween RAGE and the Wnt/β-catenin signaling pathway in this process.

Method

In this study,there were four groups, namelythe blank control group, the non-transfection group, the empty vector group, and the RAGE transfection group.Cells were co-cultured with 10 mmol/L β-glycerophosphoric acid, pyruvate and 20 mg/L AGE. The expression of osteogenic proteins in each group before and after the intervention wasdetected using Western blotting. Short interfering RNA (siRNA) targeting β-catenin was used toinhibitthe expression of β-catenin. HASMCs cultured under normal conditions were usedas the blank control.

Results

SUBMITTER: Liu Y 

PROVIDER: S-EPMC5126310 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature