Analysis of the interaction of calcitriol with the disulfide isomerase ERp57.

ABSTRACT: Calcitriol, the active form of vitamin D₃, can regulate the gene expression through the binding to the nuclear receptor VDR, but it can also display nongenomic actions, acting through a membrane-associated receptor, which has been discovered as the disulfide isomerase ERp57. The aim of our research is to identify the binding sites for calcitriol in ERp57 and to analyze their interaction. We first studied the interaction through bioinformatics and fluorimetric analyses. Subsequently, we focused on two protein mutants containing the predicted interaction domains with calcitriol: abb'-ERp57, containing the first three domains, and a'-ERp57, the fourth domain only. To consolidate the achievements we used the calorimetric approach to the whole protein and its mutants. Our results all

SUBMITTER: Gaucci E 

PROVIDER: S-EPMC5126700 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature