Identification of Novel Equine (Equus caballus) Tendon Markers Using RNA Sequencing.

ABSTRACT: Although several tendon-selective genes exist, they are also expressed in other musculoskeletal tissues. As cell and tissue engineering is reliant on specific molecular markers to discriminate between cell types, tendon-specific genes need to be identified. In order to accomplish this, we have used RNA sequencing (RNA-seq) to compare gene expression between tendon, bone, cartilage and ligament from horses. We identified several tendon-selective gene markers, and established eyes absent homolog 2 (EYA2) and a G-protein regulated inducer of neurite outgrowth 3 (GPRIN3) as specific tendon markers using RT-qPCR. Equine tendon cells cultured as three-dimensional spheroids expressed significantly greater levels of EYA2 than GPRIN3, and stained positively for EYA2 usin

SUBMITTER: Kuemmerle JM 

PROVIDER: S-EPMC5126783 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature