Epitope Identification and Application for Diagnosis of Duck Tembusu Virus Infections in Ducks.

ABSTRACT: Duck Tembusu virus (DTMUV) causes substantial egg drop disease. DTMUV was first identified in China and rapidly spread to Malaysia and Thailand. The antigenicity of the DTMUV E protein has not yet been characterized. Here, we investigated antigenic sites on the E protein using the non-neutralizing monoclonal antibodies (mAbs) 1F3 and 1A5. Two minimal epitopes were mapped to ²²¹LD/NLPW²²⁵ and ⁸⁷YAEYI⁹¹ by using phage display and mutagenesis. DTMUV-positive duck sera reacted with the epitopes, thus indicating the importance of the minimal amino acids of the epitopes for antibody-epitope binding. The performance of the dot blotting assay with the corresponding positive sera indicated that YAEYI was DTMUV type-specific, whereas ²²¹LD/NLPW<

SUBMITTER: Li C 

PROVIDER: S-EPMC5127020 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature