Multicolor Electron Microscopy for Simultaneous Visualization of Multiple Molecular Species.

ABSTRACT: Electron microscopy (EM) remains the primary method for imaging cellular and tissue ultrastructure, although simultaneous localization of multiple specific molecules continues to be a challenge for EM. We present a method for obtaining multicolor EM views of multiple subcellular components. The method uses sequential, localized deposition of different lanthanides by photosensitizers, small-molecule probes, or peroxidases. Detailed view of biological structures is created by overlaying conventional electron micrographs with pseudocolor lanthanide elemental maps derived from distinctive electron energy-loss spectra of each lanthanide deposit via energy-filtered transmission electron microscopy. This results in multicolor EM images analogous to multicolor fluorescence but with the benefit of

SUBMITTER: Adams SR 

PROVIDER: S-EPMC5127401 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature