In vivo vizualisation of mono-ADP-ribosylation by dPARP16 upon amino-acid starvation.

ABSTRACT: PARP catalysed ADP-ribosylation is a post-translational modification involved in several physiological and pathological processes, including cellular stress. In order to visualise both Poly-, and Mono-, ADP-ribosylation in vivo, we engineered specific fluorescent probes. Using them, we show that amino-acid starvation triggers an unprecedented display of mono-ADP-ribosylation that governs the formation of Sec body, a recently identified stress assembly that forms in Drosophila cells. We show that dPARP16 catalytic activity is necessary and sufficient for both amino-acid starvation induced mono-ADP-ribosylation and subsequent Sec body formation and cell survival. Importantly, dPARP16 catalyses the modification of Sec16, a key Sec body component, and we show that it is a critical event for th

SUBMITTER: Aguilera-Gomez A 

PROVIDER: S-EPMC5127640 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature