HUWE1 plays important role in mouse preimplantation embryo development and the dysregulation is associated with poor embryo development in humans.

ABSTRACT: HUWE1 is a HECT domain containing ubiquitin ligase implicated in neurogenesis, spermatogenesis and cancer development. The purpose of the current study is to investigate the role of HUWE1 in early embryo development. Here we demonstrate that Huwe1 is expressed in both nucleus and cytoplasm of preimplantation mouse embryos as well as gametes. Hypoxia (5% O₂) treatment could significantly increase Huwe1 expression during mouse embryo development process. HUWE1 knockdown inhibited normal embryonic development and reduced blastocyst formation, and increased apoptotic cell numbers were observed in the embryos of HUWE1 knockdown group. Human embryo staining result showed that reduced HUWE1 staining was observed in the poor-quality embryos. Furthermore, Western blot result showed that

SUBMITTER: Chen LJ 

PROVIDER: S-EPMC5128802 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature