P53 signaling modulation of cell cycle arrest and viral replication in porcine circovirus type 2 infection cells.

ABSTRACT: Porcine circovirus type 2 (PCV2) is a ubiquitous pathogen in the swine industry worldwide. Previous studies have shown that PCV2 infection induces host cell apoptosis through up-regulation of p53. To further identify the regulatory roles of p53 signaling in the process of PCV2 infection, we established p53 gene knockout PK15 cell lines using the genomic editor tool CRISPR/Cas9, and further investigated the roles of p53 in modulating the cell cycle and viral replication in this study. The results show that PCV2 infection induced obvious S phase accumulation in wild-type PK15 cells and a compromised S phase accumulation in the p53 gene mutation cells (813PK15 ^p53m/m ), but did not induce obvious S phase accumulation in the p53 gene knockout cells (148PK15 ^p53-/-) compar

SUBMITTER: Xu D 

PROVIDER: S-EPMC5129207 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature