The FOXD3/miR-214/MED19 axis suppresses tumour growth and metastasis in human colorectal cancer.

ABSTRACT:

Background

MiR-214 is aberrantly regulated in several tumours, but its underlying mechanisms in colorectal cancer (CRC) metastasis remain largely unknown. This study aimed to demonstrate the function and potential mechanism of miR-214 in regulating invasion and metastasis of CRC.

Methods

The transcription factor and targets of miR-214 were predicted by bioinformatics and validated using ChIP and dual-luciferase reporter assay. DNA methylation status was explored using bisulphite sequencing PCR. The in vitro and in vivo function of miR-214 in CRC was evaluated using MTT, plate colony formation, Matrigel invasion and animal models. Real-time PCR or western blotting was performed to detect FOXD3, miR-214 and MED19 expressions in CRC cells and clinical specimens.

Results

SUBMITTER: He GY 

PROVIDER: S-EPMC5129822 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature