A Regulatory Switch Alters Chromosome Motions at the Metaphase-to-Anaphase Transition.

ABSTRACT: To achieve chromosome segregation during mitosis, sister chromatids must undergo a dramatic change in their behavior to switch from balanced oscillations at the metaphase plate to directed poleward motion during anaphase. However, the factors that alter chromosome behavior at the metaphase-to-anaphase transition remain incompletely understood. Here, we perform time-lapse imaging to analyze anaphase chromosome dynamics in human cells. Using multiple directed biochemical, genetic, and physical perturbations, our results demonstrate that differences in the global phosphorylation states between metaphase and anaphase are the major determinant of chromosome motion dynamics. Indeed, causing a mitotic phosphorylation state to persist into anaphase produces dramatic metaphase-like oscillations. Th

SUBMITTER: Su KC 

PROVIDER: S-EPMC5130098 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature