PKCε as a novel promoter of skeletal muscle differentiation and regeneration.

ABSTRACT:

Introduction

Satellite cells are muscle resident stem cells and are responsible for muscle regeneration. In this study we investigate the involvement of PKCε during muscle stem cell differentiation in vitro and in vivo. Here, we describe the identification of a previously unrecognized role for the PKCε-HMGA1 signaling axis in myoblast differentiation and regeneration processes.

Methods

PKCε expression was modulated in the C2C12 cell line and primary murine satellite cells in vitro, as well as in an in vivo model of muscle regeneration. Immunohistochemistry and immunofluorescence, RT-PCR and shRNA silencing techniques were used to determine the role of PKCε and HMGA1 in myogenic differentiation.

Results

PKCε expression increases and subsequently re-localizes to the nuc

SUBMITTER: Di Marcantonio D 

PROVIDER: S-EPMC5130411 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature