Non-selective Separation of Bacterial Cells with Magnetic Nanoparticles Facilitated by Varying Surface Charge.

ABSTRACT: Recovering microorganisms from environmental samples is a crucial primary step for understanding microbial communities using molecular ecological approaches. It is often challenging to harvest microorganisms both efficiently and unselectively, guaranteeing a similar microbial composition between original and separated biomasses. A magnetic nanoparticles (MNPs) based method was developed to effectively separate microbial biomass from glass fiber pulp entrapped bacteria. Buffering pH and nanoparticle silica encapsulation significantly affected both biomass recovery and microbial selectivity. Under optimized conditions (using citric acid coated Fe₃O₄, buffering pH = 2.2), the method was applied in the pretreatment of total suspended particle sampler collected bioaerosols

SUBMITTER: Gao XL 

PROVIDER: S-EPMC5130997 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature