Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde.

ABSTRACT: Piperonal-catabolizing microorganisms were isolated from soil, the one (strain CT39-3) exhibiting the highest activity being identified as Burkholderia sp. The piperonal-converting enzyme involved in the initial step of piperonal metabolism was purified from strain CT39-3. Gene cloning of the enzyme and a homology search revealed that the enzyme belongs to the xanthine oxidase family, which comprises molybdoenzymes containing a molybdopterin cytosine dinucleotide cofactor. We found that the piperonal-converting enzyme acts on piperonal in the presence of O₂, leading to formation of piperonylic acid and H₂O₂. The growth of strain CT39-3 was inhibited by higher concentrations of piperonal in the culture medium. Together with this finding, the broad substrate

SUBMITTER: Doi S 

PROVIDER: S-EPMC5131310 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature