Centimeter-Scale Surface Interactions Using Hydrodynamic Flow Confinements.

ABSTRACT: We present a device and method for selective chemical interactions with immersed substrates at the centimeter-scale. Our implementations enable both, sequential and simultaneous delivery of multiple reagents to a substrate, as well as the creation of gradients of reagents on surfaces. The method is based on localizing submicroliter volumes of liquids on an immersed surface with a microfluidic probe (MFP) using a principle termed hydrodynamic flow confinement (HFC). We here show spatially defined, multiplexed surface interactions while benefiting from the probe capabilities such as non-contact scanning operation and convection-enhanced reaction kinetics. Three-layer glass-Si-glass probes were developed to implement slit-aperture and aperture-array designs. Analytical and numerical analysis

SUBMITTER: Taylor DP 

PROVIDER: S-EPMC5131324 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature