Ontology highlight
ABSTRACT:
SUBMITTER: Van Oss SB
PROVIDER: S-EPMC5131541 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Molecular cell 20161110 4
The five-subunit yeast Paf1 complex (Paf1C) regulates all stages of transcription and is critical for the monoubiquitylation of histone H2B (H2Bub), a modification that broadly influences chromatin structure and eukaryotic transcription. Here, we show that the histone modification domain (HMD) of Paf1C subunit Rtf1 directly interacts with the ubiquitin conjugase Rad6 and stimulates H2Bub independently of transcription. We present the crystal structure of the Rtf1 HMD and use site-specific, in vi ...[more]