The effectiveness of cooling conditions on temperature of canine EDTA whole blood samples.

ABSTRACT:

Background

Preanalytic factors such as time and temperature can have significant effects on laboratory test results. For example, ammonium concentration will increase 31% in blood samples stored at room temperature for 30 min before centrifugation. To reduce preanalytic error, blood samples may be placed in precooled tubes and chilled on ice or in ice water baths; however, the effectiveness of these modalities in cooling blood samples has not been formally evaluated. The purpose of this study was to evaluate the effectiveness of various cooling modalities on reducing temperature of EDTA whole blood samples.

Methods

Pooled samples of canine EDTA whole blood were divided into two aliquots. Saline was added to one aliquot to produce a packed cell volume (PCV) of 40% and to the

SUBMITTER: Tobias KM 

PROVIDER: S-EPMC5131627 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature