HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy.

ABSTRACT:

Objectives

The objective of this article is to investigate the contribution of colon and blood CD4 T-cell subsets expressing the chemokine receptor CCR6 to HIV persistence during antiretroviral therapy.

Design

Matched sigmoid biopsies and blood samples (n = 13) as well as leukapheresis (n = 20) were collected from chronically HIV-infected individuals receiving antiretroviral therapy. Subsets of CD4 T cells with distinct differentiation/polarization profiles were identified using surface markers as follows: memory (TM, CD45RA), central memory (TCM; CD45RACCR7), effector (TEM/TM; CD45RACCR7), Th17 (CCR6CCR4), Th1Th17 (CCR6CXCR3), Th1 (CCR6CXCR3), and Th2 (CCR6CCR4).

Methods

We used polychromatic flow cytometry for cell sorting, nested real-time PCR for HIV DNA quantific

SUBMITTER: Gosselin A 

PROVIDER: S-EPMC5131694 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature