Real-Time Visualization of Tissue Surface Biochemical Features Derived From Fluorescence Lifetime Measurements.

ABSTRACT: Fiber based fluorescence lifetime imaging has shown great potential for intraoperative diagnosis and guidance of surgical procedures. Here we describe a novel method addressing a significant challenge for the practical implementation of this technique, i.e., the real-time display of the quantified biochemical or functional tissue properties superimposed on the interrogated area. Specifically, an aiming beam (450 nm) generated by a continuous-wave laser beam was merged with the pulsed fluorescence excitation light in a single delivery/collection fiber and then imaged and segmented using a color-based algorithm. We demonstrate that this approach enables continuous delineation of the interrogated location and dynamic augmentation of the acquired frames with the corresponding fluorescence deca

SUBMITTER: Gorpas D 

PROVIDER: S-EPMC5131727 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature