Consistent quantitative gene product expression: #2. Antigen intensities on bone marrow cells are invariant between individuals.

ABSTRACT: Five reference populations in bone marrow specimens were identified by flow cytometry using specific combinations of reagents in order define the variation of gene product expression intensities both within and between individuals. Mature lymphocytes, uncommitted progenitor cells, promyelocytes, mature monocytes and mature neutrophils can be reproducibly identified as distinct clusters of events in heterogeneous, maturing bone marrow specimens. Support Vector Machines were used to identify the reference populations in order to reduce subjective bias in manually defining boundaries of these populations since they were not discretely separated from the remainder of the cells. Reference populations were identified in 50 randomly selected bone marrow aspirates obtained over a period spanning 3

SUBMITTER: Loken MR 

PROVIDER: S-EPMC5132067 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature