Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells.

ABSTRACT: Amyloidogenic protein aggregation impairs cell function and is a hallmark of many chronic degenerative disorders. Protein aggregation is also a major event during acute injury; however, unlike amyloidogenesis, the process of injury-induced protein aggregation remains largely undefined. To provide this insight, we profiled the insoluble proteome of several cell types after acute injury. These experiments show that the disulfide-driven process of nucleocytoplasmic coagulation (NCC) is the main form of injury-induced protein aggregation. NCC is mechanistically distinct from amyloidogenesis, but still broadly impairs cell function by promoting the aggregation of hundreds of abundant and essential intracellular proteins. A small proportion of the intracellular proteome resists NCC and is instea

SUBMITTER: Samson AL 

PROVIDER: S-EPMC5133435 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature