Dynamic Microenvironment Induces Phenotypic Plasticity of Esophageal Cancer Cells Under Flow.

ABSTRACT: Cancer microenvironment is a remarkably heterogeneous composition of cellular and non-cellular components, regulated by both external and intrinsic physical and chemical stimuli. Physical alterations driven by increased proliferation of neoplastic cells and angiogenesis in the cancer microenvironment result in the exposure of the cancer cells to elevated levels of flow-based shear stress. We developed a dynamic microfluidic cell culture platform utilizing eshopagael cancer cells as model cells to investigate the phenotypic changes of cancer cells upon exposure to fluid shear stress. We report the epithelial to hybrid epithelial/mesenchymal transition as a result of decreasing E-Cadherin and increasing N-Cadherin and vimentin expressions, higher clonogenicity and ALDH positive expression of

SUBMITTER: Calibasi Kocal G 

PROVIDER: S-EPMC5133540 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature