Hydrogen overproducing nitrogenases obtained by random mutagenesis and high-throughput screening.
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ABSTRACT: When produced biologically, especially by photosynthetic organisms, hydrogen gas (H2) is arguably the cleanest fuel available. An important limitation to the discovery or synthesis of better H2-producing enzymes is the absence of methods for the high-throughput screening of H2 production in biological systems. Here, we re-engineered the natural H2 sensing system of Rhodobacter capsulatus to direct the emission of LacZ-dependent fluorescence in response to nitrogenase-produced H2. A lacZ gene was placed under the control of the hupA H2-inducible promoter in a strain lacking the uptake hydrogenase and the nifH nitrogenase gene. This system was then used in combination with fluorescence-activated cell sorting flow cytometry to
SUBMITTER: Barahona E
PROVIDER: S-EPMC5133592 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
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