Relationship between nanotopographical alignment and stem cell fate with live imaging and shape analysis.

ABSTRACT: The topography of a biomaterial regulates cellular interactions and determine stem cell fate. A complete understanding of how topographical properties affect cell behavior will allow the rational design of material surfaces that elicit specified biological functions once placed in the body. To this end, we fabricate substrates with aligned or randomly organized fibrous nanostructured topographies. Culturing adipose-derived stem cells (ASCs), we explore the dynamic relationship between the alignment of topography, cell shape and cell differentiation to osteogenic and myogenic lineages. We show aligned topographies differentiate cells towards a satellite cell muscle progenitor state - a distinct cell myogenic lineage responsible for postnatal growth and repair of muscle. We analyze cell shap

SUBMITTER: Newman P 

PROVIDER: S-EPMC5133629 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature