ITRAQ-Based Quantitative Proteomic Analysis of the Potentiated and Dormant Antler Stem Cells.

ABSTRACT: As the only known organ that can completely regenerate in mammals, deer antler is of real significance in the field of regenerative medicine. Recent studies have shown that the regenerative capacity of the antlers comes from the pedicle periosteum and the cells resident in the periosteum possess the attributes of stem cells. Currently, the molecular mechanism of antler regeneration remains unclear. In the present study, we compared the potentiated and dormant antler stem cells using isobaric tags for the relative and absolute quantification (iTRAQ) labeling of the peptides, coupled with two-dimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS) to compare the proteome profiles. Proteins were identified by searching against the NCBI nr database and our own Cervine transcripto

SUBMITTER: Dong Z 

PROVIDER: S-EPMC5133779 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature