Peculiarities of the Super-Folder GFP Folding in a Crowded Milieu.

ABSTRACT: The natural cellular milieu is crowded by large quantities of various biological macromolecules. This complex environment is characterized by a limited amount of unoccupied space, limited amounts of free water, and changed solvent properties. Obviously, such a tightly packed cellular environment is poorly mimicked by traditional physiological conditions, where low concentrations of a protein of interest are analyzed in slightly salted aqueous solutions. An alternative is given by the use of a model crowded milieu, where a protein of interest is immersed in a solution containing high concentrations of various polymers that serve as model crowding agents. An expected outcome of the presence of such macromolecular crowding agents is their ability to increase conformational stability of a glob

SUBMITTER: Stepanenko OV 

PROVIDER: S-EPMC5133806 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature