Generation and Characterisation of a Reference Transcriptome for Lentil (Lens culinaris Medik.).

ABSTRACT: RNA-Seq using second-generation sequencing technologies permits generation of a reference unigene set for a given species, in the absence of a well-annotated genome sequence, supporting functional genomics studies, gene characterisation and detailed expression analysis for specific morphophysiological or environmental stress response traits. A reference unigene set for lentil has been developed, consisting of 58,986 contigs and scaffolds with an N50 length of 1719 bp. Comparison to gene complements from related species, reference protein databases, previously published lentil transcriptomes and a draft genome sequence validated the current dataset in terms of degree of completeness and utility. A large proportion (98%) of unigenes were expressed in more than one tissue, at varying levels.

SUBMITTER: Sudheesh S 

PROVIDER: S-EPMC5133886 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature