An isothermal DNA amplification method for detection of Onchocerca volvulus infection in skin biopsies.

ABSTRACT:

Background

Diagnostic procedures for the diagnosis of infection with the nematode parasite Onchocerca volvulus are currently based on the microscopic detection of microfilariae in skin biopsies. Alternative approaches based on amplification of parasitic DNA in these skin biopsies are currently being explored. Mostly this is based on the detection of the O-150 repeat sequence using PCR based techniques.

Methods

An isothermal, loop-mediated amplification method has been designed using the mitochondrial O. volvulus cox1 gene as a target.

Results

Analysis of dilution series of synthetic DNA containing the targeted sequence show a non-linear dose-response curve, as is usually the case for isothermal amplification methods. Evaluation of cross-reactivity with the heterologou

SUBMITTER: Lagatie O 

PROVIDER: S-EPMC5134125 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature