Effects of Sampling Conditions and Environmental Factors on Fecal Volatile Organic Compound Analysis by an Electronic Nose Device.

ABSTRACT: Prior to implementation of volatile organic compound (VOC) analysis in clinical practice, substantial challenges, including methodological, biological and analytical difficulties are faced. The aim of this study was to evaluate the influence of several sampling conditions and environmental factors on fecal VOC profiles, analyzed by an electronic nose (eNose). Effects of fecal sample mass, water content, duration of storage at room temperature, fecal sample temperature, number of freeze-thaw cycles and effect of sampling method (rectal swabs vs. fecal samples) on VOC profiles were assessed by analysis of totally 725 fecal samples by means of an eNose (Cyranose320^®). Furthermore, fecal VOC profiles of totally 1285 fecal samples from 71 infants born at three different hospitals wer

SUBMITTER: Berkhout DJ 

PROVIDER: S-EPMC5134625 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature