Improved B cell development in humanized NOD-scid IL2Rγ^null mice transgenically expressing human stem cell factor, granulocyte-macrophage colony-stimulating factor and interleukin-3.

ABSTRACT:

Introduction

Immunodeficient mice engrafted with human immune systems support studies of human hematopoiesis and the immune response to human-specific pathogens. A significant limitation of these humanized mouse models is, however, a severely restricted ability of human B cells to undergo class switching and produce antigen-specific IgG after infection or immunization.

Methods

In this study, we have characterized the development and function of human B cells in NOD-scid IL2Rγ^null (NSG) mice transgenically expressing human stem cell factor (SCF), granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-3 (NSG-SGM3) following engraftment with human hematopoietic stem cells, autologous fetal liver, and thymic tissues (bone marrow, liver, thymus or BLT

SUBMITTER: Jangalwe S 

PROVIDER: S-EPMC5134721 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature