Porcine Treg depletion with a novel diphtheria toxin-based anti-human CCR4 immunotoxin.

ABSTRACT: Regulatory T cells (Tregs) are known to play an important role in immunoregulation and have been shown to facilitate induction of transplantation tolerance. Chemokine (C-C motif) receptor 4 (CCR4) is expressed on the surface of effector Tregs involved in controlling alloimmune and autoimmune responses. Recently we have developed a novel diphtheria-toxin based anti-human CCR4 immunotoxin for depleting CCR4⁺ cells in vivo. In this study, we have demonstrated that the anti-human CCR4 immunotoxin bound to porcine lymphocytes including CD4⁺FoxP3⁺ Tregs. Anti-human CCR4 immunotoxin effectively depleted CCR4⁺ Foxp3⁺ porcine Tregs in vivo. We observed depletion of up to 70-85% of the CCR4⁺Foxp3⁺ porcine Tregs in the perip

SUBMITTER: Wang Z 

PROVIDER: S-EPMC5134928 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature