Isolation and Characterization of Agrobacterium Strains from Soil: A Laboratory Capstone Experience.

ABSTRACT: In this investigation, the students' goal was to isolate and characterize Agrobacterium strains from soil. Following selection and enrichment on 1A-t medium, putative Agrobacterium isolates were characterized by Gram stain reaction and biochemical tests. Isolates were further evaluated using polymerase chain reaction (PCR) with different primer sets designed to amplify specific regions of bacterial deoxyribonucleic acid (DNA). Primer sets included AGRH to identify isolates that were members of the Rhizobiaceae, BIOVAR1 primers to identify members of Agrobacterium biovar group I, and a third set, VIRG, to determine presence of virG (only present in pathogenic Agrobacterium strains). During the investigation, students applied previously learned techn

SUBMITTER: Finer KR 

PROVIDER: S-EPMC5134949 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature