DNA Methylation Signature Reveals Cell Ontogeny of Renal Cell Carcinomas.

ABSTRACT:

Purpose

DNA methylation is a heritable covalent modification that is developmentally regulated and is critical in tissue-type definition. Although genotype-phenotype correlations have been described for different subtypes of renal cell carcinoma (RCC), it is unknown if DNA methylation profiles correlate with morphological or ontology based phenotypes. Here, we test the hypothesis that DNA methylation signatures can discriminate between putative precursor cells in the nephron.

Experimental designs

We performed deep profiling of DNA methylation and transcriptome in diverse histopathological RCC subtypes and validated DNA methylation in an independent dataset as well as in The Cancer Genome Atlas Clear Cell and Chromophobe Renal Cell Carcinoma Datasets.

Results

Our data

SUBMITTER: Malouf GG 

PROVIDER: S-EPMC5135666 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature