Direct vitamin D3 actions on rhesus macaque follicles in three-dimensional culture: assessment of follicle survival, growth, steroid, and antimullerian hormone production.

ABSTRACT:

Objective

To investigate the direct actions of active 1,25-dihydroxy vitamin D3 (VD3) upon primate follicular development at specific stages of folliculogenesis.

Design

Secondary preantral follicles were isolated from rhesus monkeys ovaries, encapsulated in alginate, and cultured for 40 days. Follicles were randomly assigned to experimental groups of control, low-dose VD3 (LVD3; 25 pg/mL), and high-dose VD3 (HVD3; 100 pg/mL).

Setting

National primate research center.

Animal(s)

Adult, female rhesus macaques (Macaca mulatta).

Intervention(s)

None.

Main outcome measure(s)

Follicle survival and growth, as well as oocyte size, were assessed. Progesterone (P₄), androstenedione (A₄), E₂, and antimüllerian hormone (AMH) co

SUBMITTER: Xu J 

PROVIDER: S-EPMC5136302 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature