Genomic diversity and phylogenetic relationships of human papillomavirus 16 (HPV16) in Nepal.

ABSTRACT:

Objectives/background

Sequence variants in HPV16 confer differences in oncogenic potential; however, to date there have not been any HPV sequence studies performed in Nepal. The objective of this study was to characterize HPV16 viral genome sequences from Nepal compared to a reference sequence in order to determine their lineages. Additionally, we sought to determine if five High-grade Squamous Intraepithelial Lesion (HSIL) subjects were genetically distinct from the non-HSIL subjects.

Methods

DNA was isolated from exfoliated cervical cells from 17 individuals in Nepal who were previously identified to be HPV16-positive. A custom HPV16 Ion Ampliseq panel of multiplexed degenerate primers was designed that generated 47 overlapping amplicons and covered 99% of the viral genome

SUBMITTER: Makowsky R 

PROVIDER: S-EPMC5136510 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature