Metabarcoding of marine nematodes - evaluation of reference datasets used in tree-based taxonomy assignment approach.

ABSTRACT:

Background

Metabarcoding is becoming a common tool used to assess and compare diversity of organisms in environmental samples. Identification of OTUs is one of the critical steps in the process and several taxonomy assignment methods were proposed to accomplish this task. This publication evaluates the quality of reference datasets, alongside with several alignment and phylogeny inference methods used in one of the taxonomy assignment methods, called tree-based approach. This approach assigns anonymous OTUs to taxonomic categories based on relative placements of OTUs and reference sequences on the cladogram and support that these placements receive.

New information

In tree-based taxonomy assignment approach, reliable identification of anonymous OTUs is based on their

SUBMITTER: Holovachov O 

PROVIDER: S-EPMC5136706 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature