Digital DNA detection based on a compact optofluidic laser with ultra-low sample consumption.

ABSTRACT: DNA lasers self-amplify optical signals from a DNA analyte as well as thermodynamic differences between sequences, allowing quasi-digital DNA detection. However, these systems have drawbacks, such as relatively large sample consumption and complicated dye labelling. Moreover, although the lasing signal can detect the target DNA, it is superimposed on an unintended fluorescence background, which persists for non-target DNA samples as well. From an optical point of view, it is thus not truly digital detection and requires spectral analysis to identify the target. In this work, we propose and demonstrate an optofluidic laser that has a single layer of DNA molecules as the gain material. A target DNA produces intensive laser emission comparable to existing DNA lasers, while any unnecessary flu

SUBMITTER: Lee W 

PROVIDER: S-EPMC5137248 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature