Biliary fibrosis drives liver repopulation and phenotype transition of transplanted hepatocytes.

ABSTRACT:

Background & aims

Current research focuses on developing alternative strategies to restore decreased liver mass prior to the onset of end-stage liver disease. Cell engraftment/repopulation requires regeneration in normal liver, but we have shown that severe liver injury stimulates repopulation without partial hepatectomy (PH). We have now investigated whether a less severe injury, secondary biliary fibrosis, would drive engraftment/repopulation of ectopically transplanted mature hepatocytes.

Methods

Ductular proliferation and progressive fibrosis in dipeptidyl-peptidase IV (DPPIV)(-) F344 rats was induced by common bile duct ligation (BDL). Purified DPPIV(+)/green fluorescent protein (GFP)(+) hepatocytes were infused without PH into the spleen of BDL rats and compared to rat

SUBMITTER: Yovchev MI 

PROVIDER: S-EPMC5137249 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature