Diversity of Antiviral IgG Effector Activities Observed in HIV-Infected and Vaccinated Subjects.

ABSTRACT: Diverse Ab effector functions mediated by the Fc domain have been commonly associated with reduced risk of infection in a growing number of nonhuman primate and human clinical studies. This study evaluated the anti-HIV Ab effector activities in polyclonal serum samples from HIV-infected donors, VAX004 vaccine recipients, and healthy HIV-negative subjects using a variety of primary and cell line-based assays, including Ab-dependent cellular cytotoxicity (ADCC), Ab-dependent cell-mediated viral inhibition, and Ab-dependent cellular phagocytosis. Additional assay characterization was performed with a panel of Fc-engineered variants of mAb b12. The goal of this study was to characterize different effector functions in the study samples and identify assays that might most comprehensively and de

SUBMITTER: Huang Y 

PROVIDER: S-EPMC5137799 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature