Supramolecular polymer formation by cyclic dinucleotides and intercalators affects dinucleotide enzymatic processing.

ABSTRACT:

Background

Cyclic dinucleotides form supramolecular aggregates with intercalators, and this property could be utilized in nanotechnology and medicine.

Methods & results

Atomic force microscopy and electrophoretic mobility shift assays were used to show that cyclic diguanylic acid (c-di-GMP) forms G-wires in the presence of intercalators. The average fluorescence lifetime of thiazole orange, when bound to c-di-GMP was greater than when bound to DNA G-quadruplexes or dsDNA. The stability of c-di-GMP supramolecular polymers is dependent on both the nature of the cation present and the intercalator. C-di-GMP or cyclic diadenylic acid/intercalator complexes are more resistant to cleavage by YybT, a phosphodiesterase, than the uncomplexed nucleotides.

Conclusion

Cleavage of

SUBMITTER: Nakayama S 

PROVIDER: S-EPMC5137846 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature