Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system.

ABSTRACT: The simultaneous measurement of T cell function with recovery of individual T cells would greatly facilitate characterizing antigen-specific responses both in vivo and in model systems. We have developed a microraft array methodology that automatically measures the ability of individual T cells to kill a population of target cells and viably sorts specific cells into a 96-well plate for expansion. A human T cell culture was generated against the influenza M1p antigen. Individual microrafts on a 70 × 70 array were loaded with on average 1 CD8⁺ cell from the culture and a population of M1p presenting target cells. Target cell killing, measured by fluorescence microscopy, was quantified in each microraft. The rates of target cell death among the individual CD8⁺ T cells v

SUBMITTER: Attayek PJ 

PROVIDER: S-EPMC5138107 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature