Structure determination of a human virus by the combination of cryo-EM and X-ray crystallography.

ABSTRACT: Virus 3D atomic structures provide insight into our understanding of viral life cycles and the development of antiviral drugs. X-ray crystallography and cryo-EM have been used to determine the atomic structure of viruses. However, limited availability of biological samples, biosafety issues due to virus infection, and sometimes inherent characteristics of viruses, pose difficulties on combining both methods in determining viral structures. These have made solving the high resolution structure of some medically important viruses very challenging. Here, we describe our recently employed protocols for determining the high-resolution structure of the virus-like particle of hepatitis E virus (HEV), a pathogen of viral hepatitis in human. These protocols include utilizing recombinant baculovirus

SUBMITTER: Liu Z 

PROVIDER: S-EPMC5138254 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature