Regulation of gap junction conductance by calcineurin through Cx43 phosphorylation: implications for action potential conduction.

ABSTRACT: Cardiac arrhythmias are associated with raised intracellular [Ca²⁺] and slowed action potential conduction caused by reduced gap junction (GJ) electrical conductance (Gj). Ventricular GJs are composed of connexin proteins (Cx43), with Gj determined by Cx43 phosphorylation status. Connexin phosphorylation is an interplay between protein kinases and phosphatases but the precise pathways are unknown. We aimed to identify key Ca²⁺-dependent phosphorylation sites on Cx43 that regulate cardiac gap junction conductance and action potential conduction velocity. We investigated the role of the Ca²⁺-dependent phosphatase, calcineurin. Intracellular [Ca²⁺] was raised in guinea-pig myocardium by a low-Na solution or increased stimulation. Conduction velocity

SUBMITTER: Jabr RI 

PROVIDER: S-EPMC5138272 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature